XBP-1 Antibody [9B7E5] | PM-4937

XBP-1 Antibody [9B7E5] | PM-4937 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Mouse monoclonal XBP-1 antibody was raised against recombinant protein corresponding to amino acids 2 to 160 of human XBP-1.

Research Area: Signal Transduction

Tested Application: E, WB, ICC, IF

Application: XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.
Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 95-109 - XBP-1 Recombinant Protein

Positive Control 2: Cat. No. 17-011 - Hep G2 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: XBP-1 Monoclonal Antibody is immunoaffinity chromotography purified IgG.

Clonality: Monoclonal

Clone: 9B7E5

Isotype: IgG1, k

Conjugate: Unconjugated

Physical State: Liquid

Buffer: XBP-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: XBP-1 monoclonal antibody can be stored at -20˚C, stable for one year.

Alternate Name: XBP-1 Antibody [9B7E5] : XBP2, TREB5, XBP-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: XBP-1 Monoclonal Antibody: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER) . Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.