XIAP Antibody | 3331

XIAP Antibody | 3331 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat: (100%) , Pig: (85%) , Chicken: (91%)

Immunogen: XIAP antibody was raised against a synthetic peptide corresponding to 13 amino acids at the C-terminus of human XIAP.
The immunogen is located within amino acids 420 - 470 of XIAP.

Research Area: Apoptosis, Cancer

Tested Application: E, WB, IHC-P, IF

Application: XIAP antibody can be used for the detection of XIAP by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1324 - Human Kidney Tissue Lysate

Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 55 kDa
Observed: 53 kDa

Validation: N/A

Isoform: N/A

Purification: XIAP Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: XIAP Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: XIAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: XIAP Antibody: API3, ILP1, MIHA, XLP2, BIRC4, IAP-3, hIAP3, hIAP-3, API3, IAP3, E3 ubiquitin-protein ligase XIAP, Baculoviral IAP repeat-containing protein 4, ILP

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: XIAP Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. The X-chromosome linked inhibitor of apoptosis (XIAP) contains 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3, -7, and -9. Upregulation of XIAP expression can protect cells from apoptosis induced by low level radiation; conversely, decreased XIAP expression by antisense targeting resulted in increased cell death following low level radiation. Two negative regulators, termed XAF-1 and Smac, can bind and inhibit XIAP activity.