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XIAP E3 Ligase TR-FRET Kit | SBB-KF0057

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SKU:
SBB-KF0057-GEN
Availability:
IN STOCK
NULL964.00

Description

XIAP E3 Ligase TR-FRET Kit | SBB-KF0057

The kit uses ubiquitin labeled with either Europium-Cryptate or Cyanine5 as FRET pair donor and acceptor fluorophores respectively, completely eliminating the need for antibody based detection setups. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto XIAP leads to an increase in fluorescence emission at 665 nm (Emacceptor) and decrease at emission wavelength 620 nm (Emdonor).
South Bay Bio’s XIAP E3 Ligase TR-FRET Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto XIAP in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto XIAP in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate “true” signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.
The kit uses ubiquitin labeled with either Europium-Cryptate or Cyanine5 as FRET pair donor and acceptor fluorophores respectively, completely eliminating the need for antibody based detection setups. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto XIAP leads to an increase in fluorescence emission at 665 nm (Emacceptor) and decrease at emission wavelength 620 nm (Emdonor).

Specifications

Product Overview
Quantity: 400 wells (20μl / well)
Readout: Endpoint/Kinetic
Label or Dye: Europium Cryptate & Cy5
Detection Method: TR-FRET
Substrate Properties: Protein-Based Substrate. Typical experimental concentration is 1x.
Excitation/Emission (nm): 304/620 & 304/665
Storage Store at −80°C after product arrival. Avoid multiple freeze / thaws. It is recommended to make multiple aliquots after the first thaw.
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Additional Information

Quantity:
400 wells (20μl / well)
Readout:
Endpoint/Kinetic
Label or Dye:
Europium Cryptate & Cy5
Detection Method:
TR-FRET
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