Description
YAF2 Antibody | 28-652 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Mouse
Homology: N/A
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse YAF2.
Research Area: Transcription
Tested Application: E, WB
Application: YAF2 antibody can be used for detection of YAF2 by ELISA at 1:312500. YAF2 antibody can be used for detection of YAF2 by western blot at 0.2 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.
Specificiy: N/A
Positive Control 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 20 kDa
Validation: N/A
Isoform: N/A
Purification: Antibody is purified by peptide affinity chromatography method.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration: batch dependent
Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store YAF2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Alternate Name: YAF2, 2810021M11Rik
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Yaf2 binds to MYC and inhibits MYC-mediated transactivation. Yaf2 also binds to MYCN and enhances MYCN-dependent transcriptional activation. Yaf2 increases calpain 2-mediated proteolysis of YY1 in vitro. Yaf2 is a component of the E2F6.com-1 complex, a repressive complex that methylates Lys-9 of histone H3, suggesting that it is involved in chromatin-remodeling.