YPEL5 Antibody | 6779

YPEL5 Antibody | 6779 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Immunogen: YPEL5 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human YPEL5.
The immunogen is located within the first 50 amino acids of YPEL5.

Research Area: Cancer, Cell Cycle

Tested Application: E, WB, ICC, IF

Application: YPEL5 antibody can be used for detection of YPEL5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1288 - A20 Cell Lysate

Positive Control 2: Cat. No. 17-208 - A-20 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: YPEL5 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: YPEL5 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: YPEL5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: YPEL5 Antibody: CGI-127, Protein yippee-like 5

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: YPEL5 Antibody: YPEL5 (yippee-like 5) belongs to a family of five yippee-like proteins, all of which localize to the centrosome or mitotic spindle and are widely expressed in both adult and fetal tissue. This localization plus the fact that the family of human YPEL proteins share a high degree of sequence homology across species suggests that these proteins may have a conserved function involved in cell division. YPEL5 is expressed at the nucleus and centrosome during interphase; during mitosis, it localizes to the spindle poles, mitotic spindle, and spindle midzone during mitosis. Finally, during cytokinesis, YPEL5 is localized to the midbody. It is associated with the Ran Binding Protein in the Microtubule organizing center (RanBPM) and a related protein RanBP10.