ZAP70 Antibody | 42-992

ZAP70 Antibody | 42-992 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow

Immunogen: The immunogen for this antibody is: C-SDGYTPEPARITSPD

Research Area: Other

Tested Application: E, WB, IP

Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9kDa according to NP_001070.2) . Recommended concentration: 0.3-1ug/ml.Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from customer) .

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Specificiy: This antibody is expected to recognize isoform 1 (NP_001070.2) only.

Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Approx 70 kDa

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: ZAP70, zeta-chain (TCR) associated protein kinase 70kDa, FLJ17670, FLJ17679, SRK, STD, TZK, ZAP-70, 70 kDa zeta-associated protein, syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, zeta-chain associated protein kinase, 70kD

User Note: N/A

BACKGROUND: N/A