ZAP70 (phospho-Tyr292) polyclonal Antibody | AP0080

ZAP70 (phospho-Tyr292) polyclonal Antibody | AP0080 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Rat

Application: WB

Application Range: WB: 1:500~1:1000

Background: The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a multisubunit complex assembled from at least six different genes . The TCR subunits include the Ti a and b chains, the CD3 g, d and e chains and a z -containing homodimer or heterodimer . The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR z chain through two src-homology (SH2) domains . This binding results in the phosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr292 and Tyr319 . ZAP-70 is autophosphorylated on Tyr292, which is thought to negatively regulate ZAP-70 function in lymphocytes . Alternatively, ZAP-70 is positively regulated by phosphorylation on Tyr319, which mediates the SH2-dependent interaction between Lck and ZAP-70 .

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: p-ZAP-70 (Tyr292) polyclonal Antibody detects endogenous levels of ZAP-70 protein only when phosphorylated at Tyr292.

Molecular Weight: ~ 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase; ZAP70; SRK

Immunogen: Synthetic phosphopeptide derived from human ZAP70 around the phosphorylation site of Tyrosine 292.

Conjugate: N/A

Modification: N/A

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: N/A