ZBTB7A Antibody (C-term) [APR11233G]

ZBTB7A Antibody (C-term) [APR11233G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This ZBTB7A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 514-542 amino acids from the C-terminal region of human ZBTB7A.

Cellular Localisation: Nucleus. Note=Recruited to double-strand break sites of damaged DNA. {ECO:0000250|UniProtKB:O88939}

Molecular Weight: 61439

Clone: Polyclonal

Gene Name: ZBTB7A (HGNC:18078)

Gene ID: 51341

Function: Transcription factor that represses the transcription of a wide range of genes involved in cell proliferation and differentiation (PubMed:14701838, PubMed:17595526, PubMed:20812024, PubMed:25514493, PubMed:26455326, PubMed:26816381) . Directly and specifically binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' and represses transcription both by regulating the organization of chromatin and through the direct recruitment of transcription factors to gene regulatory regions (PubMed:12004059, PubMed:17595526, PubMed:20812024, PubMed:25514493, PubMed:26816381) . Negatively regulates SMAD4 transcriptional activity in the TGF-beta signaling pathway through these two mechanisms (PubMed:25514493) . That is, recruits the chromatin regulator HDAC1 to the SMAD4-DNA complex and in parallel prevents the recruitment of the transcriptional activators CREBBP and EP300 (PubMed:25514493) . Collaborates with transcription factors like RELA to modify the accessibility of gene transcription regulatory regions to secondary transcription factors (By similarity) . Also directly interacts with transcription factors like SP1 to prevent their binding to DNA (PubMed:12004059) . Functions as an androgen receptor/AR transcriptional corepressor by recruiting NCOR1 and NCOR2 to the androgen response elements/ARE on target genes (PubMed:20812024) . Thereby, negatively regulates androgen receptor signaling and androgen- induced cell proliferation (PubMed:20812024) . Involved in the switch between fetal and adult globin expression during erythroid cells maturation (PubMed:26816381) . Through its interaction with the NuRD complex regulates chromatin at the fetal globin genes to repress their transcription (PubMed:26816381) . Specifically represses the transcription of the tumor suppressor ARF isoform from the CDKN2A gene (By similarity) . Efficiently abrogates E2F1-dependent CDKN2A transactivation (By similarity) . Regulates chondrogenesis through the transcriptional repression of specific genes via a mechanism that also requires histone deacetylation (By similarity) . Regulates cell proliferation through the transcriptional regulation of genes involved in glycolysis (PubMed:26455326) . Involved in adipogenesis through the regulation of genes involved in adipocyte differentiation (PubMed:14701838) . Plays a key role in the differentiation of lymphoid progenitors into B and T lineages (By similarity) . Promotes differentiation towards the B lineage by inhibiting the T-cell instructive Notch signaling pathway through the specific transcriptional repression of Notch downstream target genes (By similarity) . Also regulates osteoclast differentiation (By similarity) . May also play a role, independently of its transcriptional activity, in double-strand break repair via classical non-homologous end joining/cNHEJ (By similarity) . Recruited to double-strand break sites on damage DNA, interacts with the DNA-dependent protein kinase complex and directly regulates its stability and activity in DNA repair (By similarity) . May also modulate the splicing activity of KHDRBS1 toward BCL2L1 in a mechanism which is histone deacetylase-dependent and thereby negatively regulates the pro-apoptotic effect of KHDRBS1 (PubMed:24514149) .

Summary: Tissue Location: Widely expressed (PubMed:9927193) . In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level) (PubMed:15662416) . In tonsil, expressed in squamous epithelium and germinal center lymphocytes (at protein level) (PubMed:15662416) . Up-regulated in a subset of lymphomas, as well as in a subset of breast, lung, colon, prostate and bladder carcinomas (at protein level) (PubMed:15662416) . Expressed in adipose tissues (PubMed:14701838) .

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Zinc finger and BTB domain-containing protein 7A, Factor binding IST protein 1, FBI-1, Factor that binds to inducer of short transcripts protein 1, HIV-1 1st-binding protein 1, Leukemia/lymphoma-related factor, POZ and Krueppel erythroid myeloid ontogenic factor, POK erythroid myeloid ontogenic factor, Pokemon, TTF-I-interacting peptide 21, TIP21, Zinc finger protein 857A, ZBTB7A, FBI1, LRF, ZBTB7, ZNF857A