ZC3H12A Antibody | 5673

ZC3H12A Antibody | 5673 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (82%)

Immunogen: ZC3H12A antibody was raised against a 17 amino acid synthetic peptide near the center of human ZC3H12A.
The immunogen is located within amino acids 330 - 380 of ZC3H12A.

Research Area: Innate Immunity

Tested Application: E, WB

Application: ZC3H12A antibody can be used for detection of ZC3H12A by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Specificiy: This ZC3H12A antibody is predicted to have no cross-reactivity to other members of the ZC3H12 protein family.

Positive Control 1: Cat. No. 1204 - K562 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 66 kDa
Observed: 6 kDa

Validation: N/A

Isoform: N/A

Purification: ZC3H12A Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ZC3H12A Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ZC3H12A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: ZC3H12A Antibody: MCPIP, MCPIP1, dJ423B22.1, MCPIP, Ribonuclease ZC3H12A, MCP-induced protein 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ZC3H12A Antibody: ZC3H12A, also known as MCPIP, is an essential member of a family of novel CCCH-zinc finger proteins that regulate macrophage activation and may be involved in host immunity and inflammatory diseases. ZC3H12A has RNase activity that prevents some immune disorders by directly affecting the mRNA stability of interleukins such as IL-6 and IL12p40. Mice lacking the ZC3H12A gene suffered from severe anemia, and most dies within 12 weeks. Overexpression of ZC3H12A causes ER stress and induces a number of genes involved in apoptosis and autophagy, including JNK, PUMA, and beclin-1 in cardiac myoblasts, leading to cell death. ZC3H12A can also induce adipogenesis in 3T3-L1 pre-adipocytes in the absence of PPAPgamma, demonstrating the complex roles ZC3H12A plays.