Selective antibiotic for the Sh ble gene

Zeocin^® | Selection antibiotic: endotoxin tested, sterile reagent

InvivoGen is the sole worldwide producer of Zeocin®.

Zeocin^® is a formulation of phleomycin D1, a copper-chelated glycopeptide antibiotic produced by Streptomyces CL990. Zeocin^® causes cell death by intercalating into DNA and cleaving it.
The action of Zeocin® is effective on most aerobic cells. Therefore, Zeocin® is a popular and effective antibiotic for the selection of vectors bearing the Sh ble gene in a variety of cells types (bacteria, eukaryotic microorganisms, plant and animal cells).

Resistance to Zeocin^® is conferred by the Sh ble gene product from Streptoalloteichus hindustanus, which inactivates Zeocin® upon binding to the antibiotic [1-3].
Sh ble is a small 370bp-sized gene and is carried in a number of InvivoGen’s vectors.

Typically, mammalian cells are sensitive to Zeocin^® concentrations of 50-400 µg/ml, and bacteria to 25 µg/ml.

For research use only

References:

1. Drocourt D. et al., 1990. Cassettes of the Streptoalloteichus hindustanus ble gene for transformation of lower and higher eukaryotes to phleomycin resistance. Nucl. Acids. Res. 18: 4009.
2. Gatignol A. et al., 1988. FEBS Letters. 230: 171-5.
3. Dumas P. et al., 1994. Embo J. 242 (5) 595-601.

Specifications

Product concentration: 100 mg/ml in solution

CAS number: 11006-33-0

Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.

Endotoxin level: < 1 EU/mg

Physicochemical characterization: HPLC, pH, appearance

Cell-culture tested: potency validated in Zeocin®-sensitive and Zeocin®-resistant mammalian cell lines

Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in Zeocin®-resistant cells

Contents

Zeocin® is supplied as a sterile filtered blue solution at 100 mg/ml in HEPES buffer.

This product is available in three pack sizes:

• ant-zn-1: 10 x 1 ml (1 g)
• ant-zn-5: 50 x 1 ml (5 g)
• ant-zn-5b: 1 x 50 ml (5 g)

Zeocin® is also supplied as a blue powder:

• ant-zn-1p: 1 x 1 g
• ant-zn-5p: 1 x 5 g

Zeocin® is shipped at room temperature.

Upon receipt it should be stored at 4°C or -20°C.

Zeocin® is a harmful compound. Refer to safety data sheet for handling instructions.

Details

WORKING CONCENTRATIONS

Zeocin® is normally used at a concentration of 100 μg/ml, a 1000-fold dilution from the stock solution. However, the optimal concentration needs to be determined for your cells.
Suggested concentrations of Zeocin® for selection in some examples of mammalian cells are listed below: