ZFP36 Antibody (Center) [APR14026G]

ZFP36 Antibody (Center) [APR14026G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This ZFP36 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-142 amino acids from the Central region of human ZFP36.

Cellular Localisation: Nucleus. Cytoplasm. Cytoplasmic granule. Cytoplasm, P-body. Note=Shuttles between nucleus and cytoplasm in a CRM1-dependent manner (By similarity) . Localized predominantly in the cytoplasm in a p38 MAPK- and YWHAB-dependent manner (By similarity) . Colocalizes with SH3KBP1 and MAP3K4 in the cytoplasm (PubMed:20221403) . Component of cytoplasmic stress granules (SGs) (By similarity) . Localizes to cytoplasmic stress granules upon energy starvation (PubMed:15014438) . Localizes in processing bodies (PBs) (PubMed:17369404) . Excluded from stress granules in a phosphorylation MAPKAPK2-dependent manner (By similarity) . Shuttles in and out of both cytoplasmic P-body and SGs (By similarity) {ECO:0000250|UniProtKB:P22893, ECO:0000269|PubMed:15014438, ECO:0000269|PubMed:17369404, ECO:0000269|PubMed:20221403}

Molecular Weight: 34003

Clone: Polyclonal

Gene Name: ZFP36 (HGNC:12862)

Gene ID: 7538

Function: Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE) -containing mRNA transcripts by promoting their poly (A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis (PubMed:9703499, PubMed:10330172, PubMed:10751406, PubMed:11279239, PubMed:12115244, PubMed:12748283, PubMed:15187101, PubMed:15634918, PubMed:17030620, PubMed:16702957, PubMed:20702587, PubMed:20221403, PubMed:21775632, PubMed:27193233, PubMed:23644599, PubMed:25815583, PubMed:31439631) . Acts as an 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery (PubMed:15687258, PubMed:23644599) . Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1, and hence promotes ARE-mediated mRNA deadenylation (PubMed:23644599) . Functions also by recruiting components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs (PubMed:11719186, PubMed:12748283, PubMed:15687258, PubMed:16364915) . Self regulates by destabilizing its own mRNA (PubMed:15187101) . Binds to 3'-UTR ARE of numerous mRNAs and of its own mRNA (PubMed:10330172, PubMed:10751406, PubMed:12115244, PubMed:15187101, PubMed:15634918, PubMed:17030620, PubMed:16702957, PubMed:19188452, PubMed:20702587, PubMed:20221403, PubMed:21775632, PubMed:25815583) . Plays a role in anti-inflammatory responses; suppresses tumor necrosis factor (TNF) -alpha production by stimulating ARE-mediated TNF-alpha mRNA decay and several other inflammatory ARE- containing mRNAs in interferon (IFN) - and/or lipopolysaccharide (LPS) - induced macrophages (By similarity) . Plays also a role in the regulation of dendritic cell maturation at the post-transcriptional level, and hence operates as part of a negative feedback loop to limit the inflammatory response (PubMed:18367721) . Promotes ARE-mediated mRNA decay of hypoxia-inducible factor HIF1A mRNA during the response of endothelial cells to hypoxia (PubMed:21775632) . Positively regulates early adipogenesis of preadipocytes by promoting ARE-mediated mRNA decay of immediate early genes (IEGs) (By similarity) . Negatively regulates hematopoietic/erythroid cell differentiation by promoting ARE-mediated mRNA decay of the transcription factor STAT5B mRNA (PubMed:20702587) . Plays a role in maintaining skeletal muscle satellite cell quiescence by promoting ARE-mediated mRNA decay of the myogenic determination factor MYOD1 mRNA (By similarity) . Associates also with and regulates the expression of non-ARE-containing target mRNAs at the post-transcriptional level, such as MHC class I mRNAs (PubMed:18367721) . Participates in association with argonaute RISC catalytic components in the ARE-mediated mRNA decay mechanism; assists microRNA (miRNA) targeting ARE-containing mRNAs (PubMed:15766526) . May also play a role in the regulation of cytoplasmic mRNA decapping; enhances decapping of ARE-containing RNAs, in vitro (PubMed:16364915) . Involved in the delivery of target ARE-mRNAs to processing bodies (PBs) (PubMed:17369404) . In addition to its cytosolic mRNA-decay function, affects nuclear pre-mRNA processing (By similarity) . Negatively regulates nuclear poly (A) -binding protein PABPN1-stimulated polyadenylation activity on ARE-containing pre-mRNA during LPS- stimulated macrophages (By similarity) . Also involved in the regulation of stress granule (SG) and P-body (PB) formation and fusion (By similarity) . Plays a role in the regulation of keratinocyte proliferation, differentiation and apoptosis (PubMed:27182009) . Plays a role as a tumor suppressor by inhibiting cell proliferation in breast cancer cells (PubMed:26926077) .

Summary: Tissue Location: Expressed in both basal and suprabasal epidermal layers (PubMed:27182009) . Expressed in epidermal keratinocytes (PubMed:27182009) . Expressed strongly in mature dendritic cells (PubMed:18367721) . Expressed in immature dendritic cells (at protein level) (PubMed:18367721) .

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Tristetraprolin, TTP, G0/G1 switch regulatory protein 24, Growth factor-inducible nuclear protein NUP475, Protein TIS11A, TIS11, Zinc finger protein 36 homolog, Zfp-36, ZFP36, G0S24, RNF162A, TIS11A, TTP