Description
ZIC2 Antibody | 42-349 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen: The immunogen for this antibody is: C-HSGLSSNFNEWY
Research Area: Transcription, Neuroscience
Tested Application: E, WB
Application: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:In transfected RWPE1 transiently expressing Human ZIC2-Myc a band of approx. 65kDa is observed. This band is not observed in the non-transfected RWPE1, nor when GFP is transiently expressed. The calculated molecular size is 55.0kDa according to NP_009060.2 and 58.6kDa for the ZIC2-Myc protein. Data provided by Nathan Bowen and Maryam Boseman, Clark Atlanta University, GA, USA.. We have observed an approx 75kDa band in Human Brain (Cerebellum and Cerebral Cortex) lysates Recommended concentration, 0.5-2ug/ml.
Specificiy: This antibody is expected to NOT cross-react with other ZIC proteins.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: ZIC2, Zic family member 2 (odd-paired homolog, Drosophila) , HPE5, Zic family member 2 (odd-paired Drosophila homolog) , Zinc finger protein of the cerebellum 2, zinc finger protein ZIC 2
User Note: N/A
BACKGROUND: N/A