ZIP7 Antibody | 6093

ZIP7 Antibody | 6093 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Anti-ZIP7 antibody (6093) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human ZIP7.
The immunogen is located within amino acids 20-70 of ZIP7.

Research Area: Cancer, Cell Cycle

Tested Application: E, WB, IHC-P, IF

Application: WB: 0.5-1 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate

Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 3: Cat. No. 1204 - K562 Cell Lysate

Positive Control 4: Cat. No. 1212 - 3T3 (Balb) Cell Lysate

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 50kD
Observed: 50kD

Validation:

Independent Antibody Validation in Cell lines (Figure 1) shows similar ZIP7 expression profile in human cell lines detected by two independent anti-ZIP7 antibodies that recognize different epitopes, 6093 against N-terminus domain and 24-029 against recombinant fusion protein.  ZIP7 proteins are detected in all tested cell lines at different expression levels by the two independent antibodies. 

Isoform: Human ZIP7 has one isoform (469aa, 50kD) . Mouse ZIP7 has one isoform (476aa, 51kD) and Rat ZIP7 also has one isoform (468aa, 50kD) . 6093 can detect human, mouse and rat.

Purification: ZIP7 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ZIP7 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ZIP7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: ZIP7 Antibody: KE4, HKE4, ZIP7, RING5, H2-KE4, D6S115E, D6S2244E, Zinc transporter SLC39A7, Histidine-rich membrane protein Ke4

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ZIP7 Antibody: The zinc transporter ZIP7, also known as SLC39A7, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP7 was initially identified while characterizing genes in the major histocompatibility complex on chromosome 17. ZIP7 mRNA is abundantly and widely expressed and the protein localizes to the Golgi apparatus. It functions to transport intracellular zinc from the Golgi apparatus to the cytoplasm of the cell. ZIP7 expression is expressed by zinc. ZIP7 has been suggested to act a hub for tyrosine kinase activation and may thus be a potential therapeutic target for diseases such as cancer where prevention of tyrosine kinase activation would be advantageous.