763

0.05% Trypsin Solution (with EDTA, dissolved in PBS) | EP-CM-L0437

(5 reviews) Write a Review
SKU:
763-EP-CM-L0437-GEN
£362.00

Description

0.05% Trypsin Solution (with EDTA, dissolved in PBS) | EP-CM-L0437 | Gentaur UK, US & Europe Distribution

Application: Supplements & Reagents

Media Sort: Dissociation reagent

Form: Liquid

Storage: -20°C

Shipping: Ice bag or dry ice

Expiration (month) : 12

Background: Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide chain, hydrolyze the protein between the cells, and destroy the connection between the cells, so that the tissue or the adherent cells are dispersed into a single cell. The activity of the trypsin is related to the characteristics of the tissue or the cells, the concentration of the trypsin, the temperature and the time of action. At pH 8.0 and 37°C, the effect of the trypsin is the best. Therefore, the concentration of trypsin, the temperature and the time of the pancreatic enzyme should be grasped to avoid the excessive damage of the cells. In general, the frequently-used working concentration of the trypsin is 0. 25%, while the concentration of trypsin for the semi-adherent cells or the cells is 0.05%. Since EDTA is able to bind Ca2+ and Mg2+ to destroy the cell connection to promote the dissociation of the cells, a certain amount of EDTA is often added to the trypsin solution to enhance the dissociation effect.

View AllClose

Additional Information

Size:
100 mL
View AllClose

5 Reviews

  • 4
    Original

    Posted by Jasper on 16th Sep 2022

    Gentaur provides original things to their customers and never found any complaints about their products

  • 4
    Speedy delivery

    Posted by Luis on 16th Sep 2022

    I heard rumors that they do not deliver on time but when I ordered I was quite happy because they deliver before time really happy with them and also recommend them.

  • 4
    outstanding

    Posted by Ashton on 16th Sep 2022

    their, response, shipping, and the way they deliver their products safely are outstanding

  • 4
    Lovely

    Posted by Zachary on 16th Sep 2022

    my overall interaction with Gentaur staff and its products is lovely. Feeling very happy

  • 5
    superior quality

    Posted by Milo on 16th Sep 2022

    the quality and trust they are providing to their users are superior. Really impressed

View AllClose