763

0.05% Trypsin Solution (with EDTA, phenol red, dissolved in D-Hank's) | EP-CM-L0440

(5 reviews) Write a Review
SKU:
763-EP-CM-L0440-GEN
zł1,086.00

Description

0.05% Trypsin Solution (with EDTA, phenol red, dissolved in D-Hank's) | EP-CM-L0440 | Gentaur UK, US & Europe Distribution

Application: Supplements & Reagents

Media Sort: Dissociation reagent

Form: Liquid

Storage: -20°C

Shipping: Ice bag or dry ice

Expiration (month) : 12

Background: Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide chain, hydrolyze the protein between the cells, and destroy the connection between the cells, so that the tissue or the adherent cells are dispersed into a single cell. The activity of the trypsin is related to the characteristics of the tissue or the cells, the concentration of the trypsin, the temperature and the time of action. At pH 8.0 and 37°C, the effect of the trypsin is the best. Therefore, the concentration of trypsin, the temperature and the time of the pancreatic enzyme should be grasped to avoid the excessive damage of the cells. In general, the frequently-used working concentration of the trypsin is 0. 25%, while the concentration of trypsin for the semi-adherent cells or the cells is 0.05%. Since EDTA is able to bind Ca2+ and Mg2+ to destroy the cell connection to promote the dissociation of the cells, a certain amount of EDTA is often added to the trypsin solution to enhance the dissociation effect.

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Additional Information

Size:
100 mL
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5 Reviews

  • 4
    good support

    Posted by Calvin on 16th Sep 2022

    for a company self-esteem good support is necessary and gentaur is highly appreciatable for its good support

  • 5
    superior quality

    Posted by Bentley on 16th Sep 2022

    I was not expecting such honesty in the quality of products which I got. Really superior quality of products with superior packaging

  • 4
    obrigated

    Posted by Gavin on 16th Sep 2022

    I never found any company obligated to such an extent as the gentaur is. Really a big thanks to Gentaur

  • 4
    Efficient

    Posted by Adriel on 16th Sep 2022

    from product manufacturing to delivering even in the packaging they are highly efficient and do every work with grace

  • 4
    Highly appreciate able

    Posted by Kaiden on 16th Sep 2022

    the way they are handling their management and shipping with quality is highly appreciatable

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