763

0.25% Trypsin Solution (dissolved in PBS) | EP-CM-L0446

(5 reviews) Write a Review
SKU:
763-EP-CM-L0446-GEN
€543.00

Description

0.25% Trypsin Solution (dissolved in PBS) | EP-CM-L0446 | Gentaur UK, US & Europe Distribution

Application: Supplements & Reagents

Media Sort: Dissociation reagent

Form: Liquid

Storage: -20°C

Shipping: Ice bag or dry ice

Expiration (month) : 12

Background: Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide chain, hydrolyze the protein between the cells, and destroy the connection between the cells, so that the tissue or the adherent cells are dispersed into a single cell. The activity of the trypsin is related to the characteristics of the tissue or the cells, the concentration of the trypsin, the temperature and the time of action. At pH 8.0 and 37°C, the effect of the trypsin is the best. Therefore, the concentration of trypsin, the temperature and the time of the pancreatic enzyme should be grasped to avoid the excessive damage of the cells. In general, the frequently-used working concentration of the trypsin is 0. 25%, while the concentration of trypsin for the semi-adherent cells or the cells is 0.05%. Since EDTA is able to bind Ca2+ and Mg2+ to destroy the cell connection to promote the dissociation of the cells, a certain amount of EDTA is often added to the trypsin solution to enhance the dissociation effect.

View AllClose

Additional Information

Size:
100 mL
View AllClose

5 Reviews

  • 4
    good support

    Posted by Bennett on 17th Sep 2022

    the Trust, support and relation of a company with its customers is important for its integrity and Gentaur is good in this support

  • 4
    Original

    Posted by Silas on 17th Sep 2022

    The originality of a company is shown by its product. And the products on Gentaur are highly recommendable and original

  • 4
    its good

    Posted by Kayden on 17th Sep 2022

    The company is good in its service with well-mannered workers

  • 4
    perfect

    Posted by Wesley on 17th Sep 2022

    The company is well established because of its perfect products and management

  • 4
    Excellent

    Posted by Jace on 17th Sep 2022

    The company is known for its excellent services and mannered communication with customers!

View AllClose