763

0.25% Trypsin Solution (with EDTA, dissolved in D-Hank's) | EP-CM-L0445

(5 reviews) Write a Review
SKU:
763-EP-CM-L0445-GEN
€543.00

Description

0.25% Trypsin Solution (with EDTA, dissolved in D-Hank's) | EP-CM-L0445 | Gentaur UK, US & Europe Distribution

Application: Supplements & Reagents

Media Sort: Dissociation reagent

Form: Liquid

Storage: -20°C

Shipping: Ice bag or dry ice

Expiration (month) : 12

Background: Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide chain, hydrolyze the protein between the cells, and destroy the connection between the cells, so that the tissue or the adherent cells are dispersed into a single cell. The activity of the trypsin is related to the characteristics of the tissue or the cells, the concentration of the trypsin, the temperature and the time of action. At pH 8.0 and 37°C, the effect of the trypsin is the best. Therefore, the concentration of trypsin, the temperature and the time of the pancreatic enzyme should be grasped to avoid the excessive damage of the cells. In general, the frequently-used working concentration of the trypsin is 0. 25%, while the concentration of trypsin for the semi-adherent cells or the cells is 0.05%. Since EDTA is able to bind Ca2+ and Mg2+ to destroy the cell connection to promote the dissociation of the cells, a certain amount of EDTA is often added to the trypsin solution to enhance the dissociation effect.

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Additional Information

Size:
100 mL
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5 Reviews

  • 4
    outstanding

    Posted by Emmett on 17th Sep 2022

    their customer care and delivery of their products at a time with all the details on their site is outstanding

  • 4
    Fair reasonable prices

    Posted by Evan on 17th Sep 2022

    the price of their products is very fair and reasonable according to the quality they are maintaing in their products with no contamintion

  • 5
    superior quality

    Posted by Weston on 17th Sep 2022

    I used two prodcts of Genatur and surprised by the material qulaity. That's awesome

  • 4
    superb packing

    Posted by Waylon on 17th Sep 2022

    the way they packed their products is just awesome and professional. Really impressed

  • 4
    Quick shipping

    Posted by Declan on 17th Sep 2022

    Gentaur always fulfill its promise. They always shipped their prodcuts on time without any delay. Highly recommended!

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