12S seed storage protein CRC | AS20 4403

12S seed storage protein CRC | AS20 4403 | Gentaur UK, US & Europe Distribution

Immunogen: Native, 12S globulin alpaha subunit purified from Arabidopsis thaliana and excised from SDS-PAGE gel. UniProt: Q96318, TAIR: At4g28520

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.

Format: Liquid at 2 mg/ml.

Tested Application: ELISA (ELISA), Immunolocalisation by electron microscopy (IL), Immunohistochemistry (IHC), Western blot (WB)

Related Products: collection of antibodies to vacuolar proteins

Recommended Dilutions: assay dependent (ELISA), 1: 50 (IL by electron microscopy), 1: 100 (IHC), 1: 3000 - 1: 10 000 (WB)

Molecular weight: 58 | 30 kDa (alpha subunit)

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: N/A

Background: Major 12S seed storage protein CRC (globulin) is synthesized on the endoplasmic reticulum as precursor and then transported to storage vacuoles, where it is processed at a conserved Asn-Gly peptide bond by an asparaginyl endopeptidase to produce two mature polypeptides referred to as alpha and beta subunits that are joined together by a disulfide bond. Phosphorylated in seeds on some Tyr residues in response to abscisic acid (ABA) . 12S CRC protein is cleaved to alpha and beta chains. Cellular localisation: vacuole.Alternative protein names: Cruciferin 3, Cruciferin C Legumin-type globulin Cruciferin1, Legumin-type globulin storage protein CRU1.

Reconstitution: N/A

Storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Arabidopsis thaliana

Research Area: Plant Biology