4E-T Antibody | 45-177

4E-T Antibody | 45-177 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow

Immunogen: The immunogen for this antibody is: C-AKVISVDELEYRQ

Research Area: Cancer

Tested Application: E, WB, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 140-150kDa band observed in 293 lysates (predicted size of approx. 108kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below) ) . Recommended for use at 0.25-0.5ug/mlImmunohistochemistry:In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells-. Recommended concentration, 5-10ug/ml.

Specificiy: This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1) . Reported variants represent identical protein (NP_062817.2; NP_001157973.1) .

Positive Control 1: Cat. No. 1210 - 293 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: EIF4ENIF1, 4E-T, Clast4, FLJ21601, 2610509L04Rik, eukaryotic translation initiation factor 4E nuclear import factor 1, eIF4E-transporter, FLJ26551, OTTHUMP00000063276

User Note: N/A

BACKGROUND: N/A