5-Ethynyl-2'-deoxyuridine | 9565

A novel agent for fast and sensitive detection of DNA synthesis in vivo. 5-EdU is incorporated into proliferating cells followed by detection with a fluorescent azide using click ligation. In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. May be used for studying cell proliferation in the central nervous system and may be combined with BrdU staining in a novel protocol allowing for double labeling of DNA synthesis. EdU incorporation is rapidly becoming the gold standard method for DNA labeling.

A novel agent for fast and sensitive detection of DNA synthesis in vivo. 5-EdU is incorporated into proliferating cells followed by detection with a fluorescent azide using click ligation. In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. May be used for studying cell proliferation in the central nervous system and may be combined with BrdU staining in a novel protocol allowing for double labeling of DNA synthesis. EdU incorporation is rapidly becoming the gold standard method for DNA labeling.

9565 | 5-Ethynyl-2'-deoxyuridine DataSheet

Alternate Name/Synonyms: 2′-Deoxy-5-ethynyluridine; 5-EdU

Appearance: White to off-white solid

Formulation: N/A

CAS Number: 61135-33-9

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₁₁H₁₂N₂O₅

Molecular Weight: 252.22

Cell-Permeable?: Yes

Purity: ≥98% by HPLC

Solubilities: DMSO (~10 mg/ml)

Handling: Protect from air and light

Country of Origin: USA

Tag Line: A DNA synthesis probe

MDL Number: MFCD01675687

PubChem CID: 472172

SMILES: C#CC1=CN(C(=O)NC1=O)C2CC(C(O2)CO)O

InChi: InChI=1S/C11H12N2O5/c1-2-6-4-13(11(17)12-10(6)16)9-3-7(15)8(5-14)18-9/h1,4,7-9,14-15H,3,5H2,(H,12,16,17)/t7-,8+,9+/m0/s1

InChi Key: CDEURGJCGCHYFH-DJLDLDEBSA-N