5-HT (Serotonin) 5A Receptor Antibody | 24429

5-HT (Serotonin) 5A Receptor Antibody | 24429

The ImmunoStar 5-HT5A Receptor was quality control tested using standard immunohisto-chemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/200 – 1/400 in PBS/0.3% Triton X-100 – Bn/Av-HRP. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunoblotting and immunohistochemistry. Immunoblots of rat brain extracts revealed the presence of two bands at molecular weights of 41 and 47 kD. The lower weight band agrees with the calculated molecular weight based on amino acid sequence. The higher weight may represent glycosylated receptor protein. Immunohistochemical staining of rat brain correlates well with Northern blot analysis and in situ hybridization studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT5A receptor (17-34). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat and mouse 5-HT5A receptor.

Host: Rabbit

State: Liquid

Reacts With: Clam, Rat

Availability: In Stock

Alternate Names: 5-hydroxytryptamine receptor 5A; 5-HT5A; REC17; 5ht5a,MR22; 5-hydroxytryptamine (serotonin) receptor 5A, G protein-coupled, anti-5-HT5a

RRID: AB_572213

Immunogen: Rat 5-HT5A receptor (17-34)

Gene Symbol: Htr5a