5 mg/mL MTT solution | EP-CM-L0486

5 mg/mL MTT solution | EP-CM-L0486 | Gentaur UK, US & Europe Distribution

Application: Supplements & Reagents

Media Sort: Others

Form: Liquid

Storage: -20°C, shading light

Shipping: Ice bag or dry ice

Expiration (month) : 18

Background: MTT (3- (4, 5-dimethyl-2-thiazolyl) -2, 5-diphenyl-2-H-tetrazolium bromide) is widely used for determination of cell proliferation and cytotoxicity. The MTT can be reduced by succinate dehydrogenase in mitochondria of living cells to form crystalline dark purple product formazan.Formazan was deposited in cells, but dead cells do not. The formazan can be solubilized in dimethyl sulfoxide (DMSO) .The absorbance (OD value) was measured at the wavelength of 490nm or 570nm. The number or activity of living cells could be determined by the OD value. Within a certain range of cell numbers, the amount of MTT crystal formation was proportional to the number or activity of living cells.