50mg/mL G-418 Solution | EP-CM-L0453

50mg/mL G-418 Solution | EP-CM-L0453 | Gentaur UK, US & Europe Distribution

Application: Supplements & Reagents

Media Sort: Antibiotics

Form: Liquid

Storage: -20°C, Shading light

Shipping: Ice bag or dry ice

Expiration (month) : 24

Background: G418, an aminoglycoside antibiotic, is the most commonly used resistance screening reagent for stable transfection in molecular genetic experiments.G418 can interfere with the function of 80S ribosomes and affect protein synthesis in eukaryotic cells. It is mainly used in mammalian, plant or yeast cell selection. G418 is toxic to bacteria, yeast, higher plants, mammalian cells, protozoa, and worms. Its resistance gene is a dominant phenotype, and it is located on Tn601 (903) and Tn5 transposons. Although this gene is derived from bacteria, it can also be expressed in eukaryotic cells. The introduction of resistance genes into cells results in resistance to G418, allowing cells to grow in a culture medium containing G418.This selective feature of G418 has been widely used in gene transfer, gene knockout, resistance screening and transgenic animals.