6-CFDA | 2758

6-CFDA has been used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin. CFDA enters cells and is hydrolyzed more slowly than FDA. CFDA and FDA hydrolysis obey Michaelis-Menten kinetics with Km values of around 19 and 2 µM, respectively.

6-CFDA has been used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin. CFDA enters cells and is hydrolyzed more slowly than FDA. CFDA and FDA hydrolysis obey Michaelis-Menten kinetics with Km values of around 19 and 2 µM, respectively.

2758 | 6-CFDA DataSheet

Alternate Name/Synonyms: 6-Carboxyfluorescein diacetate

Appearance: Off-white solid

Formulation: N/A

CAS Number: 3348-03-6

Structure Available?: Y

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₂₅H₁₆O₉

Molecular Weight: 460.39

Cell-Permeable?: Yes

Purity: ≥95% by HPLC

Solubilities: DMF or DMSO

Handling: Protect from air and light

Country of Origin: USA

Tag Line: A vital stain

MDL Number: MFCD00036871

PubChem CID: 151095

SMILES: CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=C(C=CC(=C5)C(=O)O)C(=O)O3

InChi: InChI=1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)

InChi Key: QMOGCCYGOPYYNT-UHFFFAOYSA-N