8-epi-PGF2α ELISA Kit - E4789

E4789 | 8-epi-PGF2α ELISA Kit DataSheet

F2αisoprostanes are a family of prostaglandin F2αlike compounds that are formed by freeαradicalαcatalyzed peroxidation of arachidonic acid. Several F2αisoprostanes, but in particular 8αepi PGF2α, are widely used as oxidative stress biomarkers. High levels of F2-IPs can disrupt the physicochemical integrity of cell membranes but more importantly can exert significant pharmacological activity. 8-epi-PGF2α is a potent vasoconstrictor acting on the renal, pulmonary, and retinal vasculatures, modulates platelet aggregation and is involved in the activation of intracellular signaling. 8-epi-PGF2α ELISA Kit is based on the Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with 8-epi-PGF2α. During the reaction, 8-epi-PGF2αin the samples or standard competes with 8-epi-PGF2α coated on the plate for binding to the anti-8-epi-PGF2αantibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microplate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of 8-epi-PGF2α. The concentration of 8-epi-PGF2αin the samples can be calculated by comparing the OD of the samples to the standard curve.

Alternate Name : 15-F2t-IsoP, 8-iso-PGF2a, 8-isoprostane, 8-iso-PG, 8-iso Prostaglandin

Tag Line : A Competitive ELISA kit for in vitro quantitative determination of 8-epi-PGF2α concentrations in serum, plasma and other biological fluids.

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Plasma, Serum, Other biological fluids

Species Reactivity : Universal

Applications : in vitro quantitative determination of 8-epi-PGF2α concentrations in serum, plasma and other biological fluids.

Features and Benefits : Precision: Coefficient of variation is < 10%., Detection Range: 15.63—1000 pg/mL, Sensitivity: 9.38 pg/mL, Specificity: This kit recognizes 8-epi-PGF2α in samples. No Significant cross-reactivity or interference between 8-epiPGF2α and analogues was observed.