ABHD5 Antibody | 45-186

ABHD5 Antibody | 45-186 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse

Homology: Expected Species Reactivity based on sequence homology: Rat, Dog

Immunogen: The immunogen for this antibody is: C-FPERPDLADQDR

Research Area: Cancer

Tested Application: E, WB, IF, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 40kDa band observed in lysates of cell lines NIH3T3/3TL (calculated MW of 39.1kDa according to NP_057090.2) . Additional faint bands of 26kDa and 18kDa were also observed, and were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.2-0.6ug/ml Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Pancreas and Kidney. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm/ vesicles of U2OS cells. Recommended concentration: 10ug/ml.

Specificiy: This antibody is not expected to cross-react with ABHD4.

Positive Control 1: Cat. No. 1202 - A431 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: ABHD5, CGI58 protein, aCGI58, bhydrolase domain containing 5, CDS, IECN2, MGC8731, NCIE2

User Note: N/A

BACKGROUND: N/A