Ac-IEPD-AFC | B2526

Ac-IEPD-AFC is a useful fluorogenic substrate for the detection of Granzyme B. Granzyme B activity can be quantified by fluorescent detection of free AFC that has an excitation maximum at 400 nm and emission maximum at 505 nm.

Ac-IEPD-AFC is a useful fluorogenic substrate for the detection of Granzyme B.

B2526 | Ac-IEPD-AFC DataSheet

Alternate Name/Synonyms: Granzyme B substrate; Ac-Ile-Glu-Pro-Asp-AFC; N-Acetyl-Ile-Glu-Pro-Asp-(7-amino-4-trifluoromethylcoumarin)

Appearance: White to off-white solid

Formulation:

CAS Number: 1135417-31-0

Structure Available?: Yes

Peptide sequence: Ac-IEPD-AFC

Salt Form: No

Molecular Formula: C₃₂H₃₈F₃N₅O₁₁

Molecular Weight: 725.67

Cell-Permeable?: True

Purity: ≥95% by HPLC

Solubilities: >14 mg/ml in DMSO

Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.

Country of Origin: USA

Tag Line: A fluorogenic Granzyme B substrate

MDL Number: MFCD03452821

PubChem CID: 25108575

SMILES: CCC(C)C(C(=O)NC(CCC(=O)O)C(=O)N1CCCC1C(=O)NC(CC(=O)O)C(=O)NC2=CC3=C(C=C2)C(=CC(=O)O3)C(F)(F)F)NC(=O)C

InChi: InChI=1S/C32H38F3N5O11/c1-4-15(2)27(36-16(3)41)30(49)38-20(9-10-24(42)43)31(50)40-11-5-6-22(40)29(48)39-21(14-25(44)45)28(47)37-17-7-8-18-19(32(33,34)35)13-26(46)51-23(18)12-17/h7-8,12-13,15,20-22,27H,4-6,9-11,14H2,1-3H3,(H,36,41)(H,37,47)(H,38,49)(H,39,48)(H,42,43)(H,44,45)/t15-,20-,21-,22-,27-/m0/s1

InChi Key: HWXKKVLJPWMVLL-FGGUPADCSA-N