ACAT-2, human recombinant | 4910

Acetyl-Coenzyme A acetyltransferase 2 (ACAT2) is an enzyme involved in lipid metabolism. Patients with ACAT2 deficiency have shown severe mental retardation and hypotonus. The ACAT2 gene shows complementary overlapping with the 3 prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.

4910 | ACAT-2 human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Acetyl-CoA acetyltransferase cytosolic, Cytosolic acetoacetyl-CoA thiolase, ACAT2, Acetyl CoA transferase-like protein, ACAT-2.

Alternates names: Acetyl-CoA acetyltransferase cytosolic, Cytosolic acetoacetyl-CoA thiolase, ACAT2, Acetyl CoA transferase-like protein, ACAT-2, Sterol O-acyltransferase 2, Acyl-coenzyme A:cholesterol acyltransferase 2, Cholesterol acyltransferase 2

Taglines: An enzyme participating in lipid metabolism

NCBI Gene ID #: 16846

NCBI Gene Symbol: LEP

Gene Source: Human

Accession #: P41160

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: 95%

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined in an in vivo assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5 µg leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 16.2 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered solution (1 mg/ml) in 10 mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% NaN3.

Reconstitution Instructions: Reconstitute in 10 mM HCl to a concentration of 1 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A