ACD1 | Accelerated cell death 1 | AS11 1783

ACD1 | Accelerated cell death 1 | AS11 1783 | Gentaur UK, US & Europe Distribution

Immunogen: Recombinant PaO from Arabidopsis thaliana Q9FYC2, At3g44880

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: collection of antibodies to proteins involved in senescencePlant protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 : 5000 (WB)

Molecular weight: 61 | 54 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: Brassica napus, Solanum lycopersicum, Nicotiana tabacum Species of your interest not listed? Contact us

Not reactive in: Pinus strobus

Additional Information: This antibody works on total cell extracts and can be used as a senescence marker. Predicted size of Acd1 precursor protein is about 61 kD including the transit peptide, but it must be processed to a smaller size. Using fresh extracts is recommended to decrease possible cross-reaction with Rubisco.

Background: Acd1 (accelerated cell death 1) EC=1.14.12.20 is an enzyme involved in chlorophyll breakdown, pheophorbide a oxygenase. This enzyme seems to induce cell death in Arabidopsis leaves in the dark. Synonymes:PaO, Lls1, lethal leaf-spot 1 homolog, pheide a oxygenase

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT3G44880

Category: Senescence/Cell death

Research Area: Cell Signaling, Apoptosis