Acetylated Lysine (monoclonal, clone 7F8) | AS10 707-100

Acetylated Lysine (monoclonal, clone 7F8) | AS10 707-100 | Gentaur UK, US & Europe Distribution

Immunogen: acetylated KLH

Host: Mouse

Conjugation: N/A

Clonality: Monoclonal

Isotype: IgG1

Purity: Total IgG fraction. Protein G purified.

Format: Liquid

Tested Application: ELISA (ELISA), Immunocytochmistry/Immunofluorescence (ICC/IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)

Related Products: AS15 3018 | Anti-Acetylated Lysine, rabbit antibodiesAS10 707-100 | Anti-Acetylated lysine (100 µl), mouse antibodiesAS15 3019 | Anti-Acetylated Lysine, APL conjugated, rabbit antibodiesAS15 3020 | Anti-Acetylated Lysine, FITC conjugated, rabbit antibodiesSecondary antibodies

Recommended Dilutions: 1 : 100 (IHC), 1 : 1000 (WB)

Molecular weight: N/A

Confirmed Reactivity: Bovine, avian

Predicted Reactivity: Higher plants

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: 1 µg of this antibody is sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein and ECL detection system

Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1) . The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2) . Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3) .

Reconstitution: N/A

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Translation

Research Area: Epigenetics