ACK1, Human Recombinant | P1499

Activated CDC42 kinase 1, also known as ACK1, is an enzyme that is encoded by the TNK2 gene in humans. TNK2 gene encodes a non-receptor tyrosine kinase, ACK1, that binds to multiple receptor tyrosine kinases e.g. EGFR, MERTK, AXL, HER2 and insulin receptor (IR). ACK1 also interacts with Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain.

P1499 | ACK1, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: Tyrosine kinase non-receptor protein 2, CDC42 kinase 1, TNK2

Alternates names: Tyrosine kinase non-receptor protein 2, CDC42 kinase 1, TNK2

Taglines: Non-receptor tyrosine-protein kinase that is implicated in cell migration, survival, growth and proliferation

NCBI Gene ID #: 10188

NCBI Gene Symbol: TNK2

Gene Source: Human

Accession #: Q07912

Recombinant: True

Source: Insect Cells (sf9)

Purity by SDS-PAGE: >95%

Assay: SDS-PAGE

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): The specific activity is ≥ 500 U/mg. Specific activity was tested using ADPsensor™ Universal Kinase Activity Assay Kit (Cat# K212).

Biological activity:

Results:

Binding Capacity:

Unit Definition: One unit of ACK1 is the amount of enzyme that generates 1.0 umol of ADP per min at pH 7.2 at 37°C using a poly (Glu:Tyr, 4:1) synthetic peptide as substrate.

Molecular Weight: 43.5 KDa (aa 110-476, N-His tag)

Concentration:

Appearance: Liquid

Physical form description: Liquid

Reconstitution Instructions:

Amino acid sequence: aa 110-476

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans