ACLY Antibody | 15-947

ACLY Antibody | 15-947 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 752-1101 of human ACLY (NP_001087.2) .

Research Area: Cancer, Signal Transduction

Tested Application: WB

Application: WB: 1:200 - 1:2000

Specificiy: N/A

Positive Control 1: HepG2

Positive Control 2: HeLa

Positive Control 3: Raji

Positive Control 4: NIH/3T3

Positive Control 5: Mouse brain

Positive Control 6: Mouse heart

Molecular Weight: Observed: 120kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: ACL, ATPCL, CLATP, ATP-citrate synthase, ATP-citrate (pro-S-) -lyase, citrate cleavage enzyme

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440, 000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.