ACO1 Antibody | 29-285

ACO1 Antibody | 29-285 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Rat

Homology: N/A

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ACO1.

Research Area: Cancer

Tested Application: E, WB, IHC

Application: ACO1 antibody can be used for detection of ACO1 by ELISA at 1:62500. ACO1 antibody can be used for detection of ACO1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.

Specificiy: N/A

Positive Control 1: Cat. No. XBL-10408 - Fetal Kidney Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 98 kDa

Validation: N/A

Isoform: N/A

Purification: Antibody is purified by peptide affinity chromatography method.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Concentration: batch dependent

Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store ACO1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Alternate Name: ACO1, IRP1, ACONS, HEL60, IREB1, IREBP, IREBP1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ACO1, also known as iron regulatory element binding protein 1 (IREB1) , is a cytosolic protein which binds to iron-responsive elements (IREs) . It plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1) , is a cytosolic protein which binds to iron-responsive elements (IREs) . IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.