ACP5/TRAP, human recombinant | P1055

ACP5, also known as tartrate-resistant acid phosphatase type 5, is involved in osteopontin and bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Recombinant human ACP5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

P1055 | ACP5/TRAP human recombinant DataSheet

Biomolecule/Target: ACP5

Synonyms: Tartrate-resistant acid phosphatase type 5, SPENCDI, TRAP

Alternates names: Tartrate-resistant acid phosphatase type 5, SPENCDI, TRAP

Taglines: An enzyme expressed in bone-resorbing osteoclasts and involved in osteopontin/bone sialoprotein dephosphorylation.

NCBI Gene ID #: 16846

NCBI Gene Symbol: LEP

Gene Source: Human

Accession #: P41160

Recombinant: Yes

Source: Baculovirus

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its abolity to hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.

Biological activity: Determined in an in vivo assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5 µg leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.

Results: > 5,000 units/mg

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.

Molecular Weight: 16.2 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.

Reconstitution Instructions: Reconstitute in 10 mM HCl to a concentration of 1 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A