Active AHCY, human recombinant | 7527

AHCY (EC 3.3.1.1) is an enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). AHCY controls the intracellular S-adenosylhomocysteine (SAH) concentration that is crucial for transmethylation reactions. AHCY deficiency causes hypermethioninemia. The recombinant human AHCY produced in E.Coli is a single, non-glycosylated polypeptide chain and is purified by proprietary chromatographic techniques.

7527 | Active AHCY human recombinant DataSheet

Biomolecule/Target: AHCY/SAHH

Synonyms: SAHH, AdoHcyase, S-adenosyl-L-homocysteine hydrolase, Adenosyl Homocysteinase

Alternates names: SAHH, AdoHcyase, S-adenosyl-L-homocysteine hydrolase, Adenosyl Homocysteinase

Taglines: Catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy).

NCBI Gene ID #: 25608

NCBI Gene Symbol: LEP

Gene Source: Human

Accession #: P50596

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED determined by the leptin-dependent stimulation of human OB-R transfected murine BaF3 indicator cells is 0.35-0.06 ng/ml.

Results: ’s Active AHCY has been tested for its activity using Adenosyl Homocysteinase (AHCY) Activity Fluorometric Assay Kit (K807-100). It has a specific activity of >350 mU/mg.

Binding Capacity: N/A

Unit Definition: One unit of AHCY activity is the amount of enzyme that hydrolyzes the substrate to yield 1 µmol of adenosine/min at 37°C.

Molecular Weight: 16.2 kDa

Concentration: 5 mg/ml

Appearance: Liquid

Physical form description: 5 mg/ml in 50 mM potassium phosphate, 1 mM EDTA, pH 7.2 containing 20% glycerol.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1–1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for three months. For long-term storage it is recommended to add a carrier protein (0.1% HAS or BSA).

Amino acid sequence: N/A