Active Caspase-1, rat recombinant | 7524

Caspase-1 (also known as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kDa) and small (10 kDa) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant rat caspase-1 was expressed in E. coli as a single polypeptide of 284 amino acids with molecular weight of 34.6. The caspase-1 is purified and activated by proprietary techniques.

7524 | Active Caspase-1 rat recombinant DataSheet

Biomolecule/Target: Caspase-1

Synonyms: Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, IL-1BC, p45.

Alternates names: Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, IL-1BC, p45.

Taglines: A cysteine protease involved in apoptosis

NCBI Gene ID #: 8744

NCBI Gene Symbol: TNFL9

Gene Source: Rat

Accession #: P41273

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The ED for the effect is less than 10 ng/ml. Please note: results may vary with PBMC donors.

Results: >3000 units/mg

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19.5 kDa

Concentration: N/A

Appearance: Semi-Dry

Physical form description: Semi-Dry

Reconstitution Instructions: We recommend a quick spin followed by reconstitution in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A