Active D-2-Hydroxyglutaratate Dehydrogenase (D2HGDH), Recombinant | P1001

D-2-hydroxyglutarate (D2HG) level is significantly increased in metabolic diseases and various cancers such as acute myeloid leukemia. Studies suggest that the detection of D2HG serves as a biomarker assay related to IDH (isocitrate dehydrogenase) mutations. D2HGDH is a special NAD-dependent enzyme which reacts with D2HG specifically and converts D2HG to -ketoglutarate. D2HGDH is a key enzyme to distinguish between two metabolites, D2HG and L-2-hydroxyglutarate (L2HG), during biomarker assays.

P1001 | Active D-2-Hydroxyglutaratate Dehydrogenase D2HGDH Recombinant DataSheet

Biomolecule/Target: D2HGDH

Synonyms: D-2-hydroxyglutarate dehydrogenase, D2HGDH, D2HGD

Alternates names: D-2-hydroxyglutarate dehydrogenase, D2HGDH, D2HGD

Taglines: D2HGDH is an active protein that catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.

NCBI Gene ID #: 3604

NCBI Gene Symbol: TNR9

Gene Source: Acidaminococcus fermentans

Accession #: Q07011

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): The specific activity of D2HGDH is 90,000 mU/mg. (Specific activity is tested by the D-2-Hydroxyglutarate Colorimetric Assay Kit (Cat. No. 213-100) (Unit Definition: One unit is the amount of enzyme that reduces 1.0 µmole of NAD+ to NADH per min. at pH 8 at 37°C.)

Biological activity: Determined by its inhibitory effect of the 4-1BBL mediated stimulation of IL-8 production by human PBMC. About 90% of inhibition was seen using a concentration of 1 µg for both 4-1BBL and 4-1BBR.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17.8 kDa

Concentration: N/A

Appearance: Dry powder

Physical form description: Lyophilized powder

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A