Active HRV14 3C Protease Recombinant | 8012

Human rhinovirus (HRV) infections are the most frequent causative agents of common cold and various other upper respiratory tract infections. Rhinoviruses are members of the picornavirus family, which have a positive-sense, single-stranded RNA genome that is translated into a single polyprotein precursor. In the case of HRVs, the viral polyprotein is mainly processed by the proteases (2A and 3C) to generate functional proteins and enzymes. ’s active HRV14 3C Protease is a recombinant form of the 3C protease from human rhinovirus type 14. The enzyme is highly pure with selective and high site-specific cleavage activity for Precission cleavage site (Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro), thereby allowing for the production of recombinant proteins with minimal number of extra amino acids. HRV14 3C Protease also has therapeutic implications as a potential target for the development of antiviral agents.

8012 | Active HRV14 3C Protease Recombinant DataSheet

Biomolecule/Target: HRV14 3C Protease

Synonyms: Picornain 3C, P3C

Alternates names: Picornain 3C, P3C

Taglines: 90% Pure Active Recombinant HRV14 3C Protease for inhibitor screening assays, activity studies, selectivity profiling, western blotting, ELISA, and numerous such applications.

NCBI Gene ID #: 1673

NCBI Gene Symbol: DEFB2

Gene Source: HRV14

Accession #: O15263

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): ’s Active HRV14 3C Protease has been tested for its activity using HRV 3C Protease Activity Assay Kit (Colorimetric) (K214-100).

Biological activity: Determined by its ability to chemoattract immature human dendritic cells using a concentration of 10-100 ng/ml.

Results: ’s Active HRV14 3C Protease has been tested for its activity using HRV 3C Protease Activity Assay Kit (Colorimetric) (K214-100).

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 4.3 kDa

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml in 50 mM Tris, 100 mM NaCl, 5 mM DTT, pH 8.0 containing 20% glycerol

Reconstitution Instructions: Reconstitute in ddHO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers or store d at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A