Active MANF, Human Recombinant | P1706

MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote the survival, growth and function of dopamine-specific neurons. MANF can prevent 6-OHDA-induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson's disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress.

P1706 | Active MANF, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: arginine-rich, mutated in early stage tumors; Protein ARMET Mesencephalic Astrocyte-derived Neurotrophic Factor, ARMET, Arginine-rich protein (ARP)

Alternates names:

Taglines: A secreted neurotrophic factor that is mutated in a variety of tumors

NCBI Gene ID #: 7873

NCBI Gene Symbol:

Gene Source: Human

Accession #: P55145

Recombinant: True

Source: E.coli

Purity by SDS-PAGE: ≥ 98% by SDS-PAGE gel and HPLC analyses

Assay:

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method):

Biological activity: Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 15-25 ug/ml.

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: 18.1 kDa

Concentration:

Appearance: Lyophilized powder

Physical form description: Lyophilized protein powder

Reconstitution Instructions: Reconstitute to desired concentration using sterile water

Amino acid sequence: LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans