Active Recombinant Human beta-Secretase 1 - BACE-1 | 7609

-Secretase, also called BACE1 (-site of APP cleaving enzyme) or memapsin-2 is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells. The transmembrane protein, contains two active site aspartate residues in its extracellular protein domain and may function as a dimer..

7609 | Active Recombinant Human beta-Secretase 1 - BACE-1 DataSheet

Biomolecule/Target: N/A

Synonyms: Beta-Secretase

Alternates names: Beta-secretase 1, Aspartyl protease 2, ASP2, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2, Aspartic-like protease 56 kDa, Aspartyl protease 1, Beta-site amyloid precursor protein cleaving enzyme 2, Down region aspartic protease, Memapsin-1, Membrane-associated aspartic protease 1, Theta-secretase

Taglines: A protease important in the formation of myelin sheaths in PNC.

NCBI Gene ID #: 10148

NCBI Gene Symbol: EBI3

Gene Source: Human

Accession #: NP_005746

Recombinant: Yes

Source: Insect cell

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 120x103 units/mg

Biological activity: Assay data for human recombinant EBI-3 is based upon qualitative binding to anti-EBI-3 antibody.

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that produces 1 pmol/min EDANS by hydrolyzing the substrate BVT9964 (NH2-Arg-Glu(EDANS)-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)-Arg-COOH) at 37°C in 100 mM acetate buffer pH 4.0.

Molecular Weight: 23.3 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Supplied as a lyophilized powder.

Reconstitution Instructions: When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile HO at a concentration of 0.1 – 0.5 mg/ml, which can be further diluted into other aqueous solutions.

Amino acid sequence: N/A