Active Recombinant Human CAXII Protein | P1704

CA12, also known as Carbonic anhydrase 12, is an enzyme that in humans is encoded by the CA12 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas.

Biomolecule/Target :

Synonyms: Carbonic anhydrase 12 isoform 1, CAXII, HsT18816, CA12, FLJ20151, Carbonate dehydratase XII, carbonic anhydrase XII, CA-XII, Tumor antigen HOM-RCC-3.1.3

Alternates names:

Taglines: Overexpressed in renal carcinomas

NCBI Gene ID #: 771

NCBI Gene Symbol:

Gene Source: Human

Accession #: O43570

Recombinant: False

Source: Baculovirus

Purity by SDS-PAGE: ≥ 90% by SDS-PAGE

Assay:

Purity:

Assay #2:

Endotoxin Level: < 1 EU per 1 ug of protein as determined by LAL method

Activity (Specifications/test method): Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyzes 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37 °C.

Biological activity:

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: 31.94 kDa

Concentration: 0.5 mg/mL

Appearance: Liquid

Physical form description: Liquid

Reconstitution Instructions:

Amino acid sequence: The target is expressed with the sequence (aa 25-301) of Human CAXII with a His-tag at the C-terminus.

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans