Active sFRP-4, Human Recombinant | P1709

Secreted Frizzled-Related Proteins (sFRPs) are a family of glycosylated Wnt antagonists characterized by a conserved cysteine-rich domain that shares homology with the cysteine-rich, extracellular domain Frizzled proteins use for the binding of Wnt proteins and receptors. Lacking the transmembrane and intracellular domains of the Frizzled proteins, sFRPs function as soluble modulators of the Wnt signaling pathway through the direct binding of Wnt proteins to this cysteine-rich domain, and the resultant inhibition of Wnt receptor binding and signaling capabilities. sFRP-4 is widely distributed in a variety of embryonic and adult tissues where it can function as a circulating antiangiogenic factor, a potent proapoptotic factor, an inhibitor of insulin secretion, and a suppressor of both tumor growth and metastatic potential through disruption of the Wnt signaling pathway. Research has demonstrated the existence of a direct correlation between the downregulation and/or absence of circulating sFRP-4 and the progression of several cancer types, including ovarian, endometrial, prostate and lung. Upregulation of circulating sFRP-4 has been linked to the deterioration of glucose metabolism in the case of type 2 diabetes, as well as the suppression of the keratinocyte hyperproliferation and epidermal hyperlasia that are definitive of psoriasis.

P1709 | Active sFRP-4, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: Secreted frizzled-related protein 4; DDC-4 SFRP4, FRP-4, FRPHE, PYL Frizzled protein, human endometrium; FRP-AP; frpHE; FRPHEMGC26498; FrzB2

Alternates names:

Taglines: A soluble modulator of Wnt signaling

NCBI Gene ID #: 6424

NCBI Gene Symbol:

Gene Source: Human

Accession #: Q6FHJ7

Recombinant: True

Source: CHO cells

Purity by SDS-PAGE: ≥ 95% by SDS-PAGE gel and HPLC analyses

Assay:

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method):

Biological activity: Determined by its ability to decrease alkaline phosphatase activity in CCL-226 cells when treated with 25 ng/ml of Murine Wnt-3a.

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: 37.8 kDa

Concentration:

Appearance: Lyophilized protein

Physical form description: Lyophilized protein powder

Reconstitution Instructions: Reconstitute to desired concentration using sterile water.

Amino acid sequence: The target is expressed with the sequence (Val 19 to Val 346) of Human sFRP-4.

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans