Active SIRT2, human recombinant | 7698

Sirtuins function as intracellular regulatory proteins. Human Sirtuin 2 is a member of the class III histone deacetylases (HDACs) and has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging. Human SIRT2 is a cytoplasmic protein responsible for the deacetylation of histone H4 and a-tubulin, a modification important for controlling the cell cycle. Specifically, SIRT2 protein co-localizes with HDAC6 and microtubules and functions as a mitotic checkpoint in preventing chromosomal instability that can lead to hyperploid cells. SIRT2 is found in many tissues, but is specifically enriched in skeletal muscle, the heart, and in oligodendroglia cells in the brain. The enzymatic activity of class III HDACs is nicotinamide adenine dinucleotide (0.1mM NAD+) dependent and insensitive to HDAC inhibitor trichostatin A.

7698 | Active SIRT2 human recombinant DataSheet

Biomolecule/Target: SIRT2

Synonyms: NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIR2L, SIR2L2.

Alternates names: NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIR2L, SIR2L2.

Taglines: Human SIRT2 from E.Coli, AOF, 90% pure, biologically active.

NCBI Gene ID #: 10148

NCBI Gene Symbol: EBI3

Gene Source: Human

Accession #: NP_005746

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): Activity of SIRT2 was tested with ’s Sirtuin Activity assay kit, Cat # K324-100, using the substrate Arg-His-Lys-Lys(Ac)-AFC, which is a fluorogenic, acetylated peptide based on residues 379-382 of p53.

Biological activity: Assay data for human recombinant EBI-3 is based upon qualitative binding to anti-EBI-3 antibody.

Results: 4 mU/mg

Binding Capacity: N/A

Unit Definition: One unit of the recombinant SIRT2 is defined as the amount of enzyme that deacetylates 1 µmol of substrate Arg-His-Lys-Lys(Ac)-AFC per minute at 37°C in a reaction solution using Sirtuin Activity assay kit (Cat # K324-100).

Molecular Weight: 23.3 kDa

Concentration: N/A

Appearance: Sirtuin 2 (SIRT2) with a His-tag is supplied as lyophilized powder.

Physical form description: Human Sirtuin 2 is lyophilized from 50 mM Tris, pH 8.0.

Reconstitution Instructions: When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile HO at a concentration of 0.1 – 0.5 mg/ml, which can be further diluted into other aqueous solutions.

Amino acid sequence: N/A