Active SIRT7, human recombinant | 7846

The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group. There are seven human sirtuins, which have been designated SIRT1-SIRT7. Recently, SIRT7 has been shown to activate transcription by RNA polymerase I and deacetylate p53. SIRT7 prevents progressive functional deterioration of the heart, and is suggested to play an important role in regulation of stress responses and cell death in the heart.

7846 | Active SIRT7 human recombinant DataSheet

Biomolecule/Target: SIRT7

Synonyms: NAD-dependent deacetylase 7; SIR2-like protein 7; Sirtuin 7; SIR2L7; Silent Information Regulator 7

Alternates names: NAD-dependent deacetylase 7; SIR2-like protein 7; Sirtuin 7; SIR2L7; Silent Information Regulator 7

Taglines: Intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.

NCBI Gene ID #: 50498

NCBI Gene Symbol: EBI3

Gene Source: Human

Accession #: Q3U1K3

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): ’s Active His-tagged SIRT7 has been tested for its activity using Sirtuin Activity Assay Kit (K324-100). It has a specific activity of >1 µU/mg.

Biological activity: Assay data for mouse recombinant EBI-3 is based upon qualitative binding to anti-EBI-3 antibody.

Results: >1 µU/mg

Binding Capacity: N/A

Unit Definition: One unit of Sirtuin activity is the amount of enzyme that deacetylates an acetylated p53-AFC substrate to finally yield 1 µmol of AFC/min at 37°C.

Molecular Weight: 22.9 kDa

Concentration: 2 mg/ml

Appearance: Liquid

Physical form description: 2 mg/ml in 50 mM Tris, pH 8.0, 100 mM NaCl, containing 5 mM DTT and 20% glycerol.

Reconstitution Instructions: When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile HO at a concentration of 0.1 – 0.5 mg/ml, which can be further diluted into other aqueous solutions.

Amino acid sequence: N/A