Active Transglutaminase 2 (His-tagged), human recombinant | 7700

Tissue transglutaminase, a 78-kDa calcium dependent enzyme (EC 2.3.2.13), is found both in the intracellular and the extracellular spaces of various types of tissues. TG2 crosslinks proteins between the -amino group of a lysine residue and the -carboxamide group of glutamine residue, creating an inter- or intramolecular bond that is highly resistant to proteolysis (protein degradation). TG2 also possesses deamidation, GTP-binding/hydrolyzing, and isopeptidase activities. Intracellular TG2 is thought to play an important role in apoptosis, while extracellular TG2 has been linked to cell adhesion, ECM stabilization, wound healing, receptor signaling, cellular proliferation, and cellular motility.

7700 | Active Transglutaminase 2 His-tagged human recombinant DataSheet

Biomolecule/Target: Transglutaminase 2

Synonyms: TG2, tTG, tissue transglutaminase, transglutaminase C, TGC, protein-glutamine-gamma-glutamyltransferase, TGM2

Alternates names: TG2, tTG, tissue transglutaminase, transglutaminase C, TGC, protein-glutamine-gamma-glutamyltransferase, TGM2

Taglines: His tagged Human Transglutaminase 2 from E.Coli, AOF, 90% pure, biologically active.

NCBI Gene ID #: 5473

NCBI Gene Symbol: NAP2

Gene Source: Human

Accession #: P02775

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): ’s Active His-tagged TG2 has been tested for its activity. It has a specific activity of >400 mU/mg. 1 U of TG2 activity is the amount of enzyme that will catalyze the formation of 1 mol of hydroxamate per min as measured using Transglutaminase Activity Assay Kit (Colorimetric) ( Cat # K571-100).

Biological activity: Determined by its ability to chemoattract human neutrophils using a concentration range of 1.0-10 ng/ml.

Results: >400 mU/mg

Binding Capacity: N/A

Unit Definition: 1 U of TG2 activity is the amount of enzyme that will catalyze the formation of 1 mol of hydroxamate per min as measured using Transglutaminase Activity Assay Kit (Colorimetric) ( Cat # K571-100).

Molecular Weight: 7.6 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized from 5 mg/ml solution in a proprietary buffer.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20° for future use.

Amino acid sequence: N/A