ACTIVITY Aggrecanase Assay

ACTIVITY Aggrecanase Assay | 30510111 | 619

Product Features:

Type: ELISA (Activity)

Reactivity : Aggrecanase

Antigen : Aggrecanase

Detection Range : 0.024 - 1.5 nM ADAMTS4

Application : The assay measures activity of aggrecanases. It is used to screen and characterize aggrecanase inhibitors.

Description : The assay consists of two moduls, the Aggrecanase Modul and the ELISA Module. A recombinant fragment of human aggrecan inter-globular domain (aggrecan-IGD) is first digested with aggrecanase. Proteolytic cleavage of the substrate releases an aggrecan peptide with the N-terminal sequence ARGSVIL (ARGSVIL-peptide). Subsequently, ARGSVIL-neoepitope containing cleavage products are detected and quantified by ELISA using two monoclonal anti-peptide antibodies .

Research Area : Cancer, Rheumatic Diseases, Arthritis, Cardiovascular, Cell Biology, Signal Transduction, Immunology

Specificity : Aggrecanase

Immunogen : N/A

Sensitivity : 12 pM ADAMTS4

Predicted Reactivity : Aggrecanase

Cross Reactivity : N/A

Minimum Shelf life time in months : 6 months

Storage Shipping Temp Min : dry Ice and ice pack

Storage Shipping Temp Max : dry Ice and ice pack

Storage Long Term : -20 °C and 2-8 °C

Principle : Proteolytic Reaction and Sandwich-ELISA

Components : microplate, buffers, antibody conjugate,standards

Characteristics : Kit

Sample Type :

Format : 96 well microplate