ACTN1 Antibody | 14-062

ACTN1 Antibody | 14-062 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Monkey

Homology: N/A

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ACTN1 (NP_001123477.1) .

Research Area: Apoptosis, Cell Cycle, Immunology, Signal Transduction, Stem Cell

Tested Application: WB, IHC

Application: WB: 1:500 - 1:2000
IHC: 1:50 - 1:200

Specificiy: N/A

Positive Control 1: COS-7

Positive Control 2: PC-3

Positive Control 3: U-251MG

Positive Control 4: A673

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Observed: 105kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: ACTN1, FLJ40884, BDPLT15

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.