ADAM17 (Human) ELISA Kit | E5105-100

ADAM17 (Human) ELISA Kit | E5105-100

Description: ADAM17 is a member of the ADAM (a disintegrin and metalloproteinase) gene family and is involved in many pathological processes such as cancer, inflammatory diseases, neurological diseases, cardiovascular diseases, atherosclerosis, diabetes, and hypertension. ADAM17 may play a prominent role in the Notch signaling pathway, during the proteolytic release of the Notch intracellular domain (from the Notch1 receptor) that occurs following ligand binding. It also regulates the MAP kinase signaling pathway by regulating shedding of the EGFR ligand amphiregulin in the mammary gland. ADAM17 also has a role in the shedding of L-selectin, a cellular adhesion molecule. ADAM17 inhibition attenuates fibrosis and inflammation, suggesting that its inhibition may be a possible new valuable therapeutic tool in fibrotic kidney disease treatment. ADAM17 also has a role in the formation and progression of different tumor entities. ’s ADAM17 uman) ELISA Kit is used for the quantitative detection of ADAM17 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of ADAM17 in the sample.

Alternate Name: TACE ELISA Kit; TNF α Converting Enzyme ELISA Kit; ADAM17 ELISA Kit; CD156b ELISA Kit; CSVP ELISA Kit; ADAM18 ELISA Kit; CD156b antigen ELISA Kit; ADAM metallopeptidase domain 18 ELISA Kit; disintegrin and metalloproteinase domain-containing protein 17 ELISA Kit; Snake venom-like protease ELISA Kit; TNF-alpha convertase ELISA Kit TNF-alpha converting enzyme ELISA Kit; TNF-alpha-converting enzyme ELISA Kit

Tag Line: A highly sensitive sandwich ELISA kit for the quantitative measurement of ADAM17

Summary: N/A

Detection Method: Absorbance is measured at 450 nm

Sample Type: Human Serum, Plasma, Tissue lysates and other biological fluids

Species Reactivity: Human

Applications: The ELISA kit is used for the quantitative detection of ADAM17 in serum, plasma, tissue homogenates and other biological fluids

Features & Benefits: Recovery Rate: 86-97% for serum, 86-101% for EDTA plasma and 85-100% for heparin plasma Highly sensitive and specific Sensitivity: 37.5 pg/ml Detection range: 62.5-4000 pg/ml Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%