ADAM33 (Human) ELISA Kit | E5102-100

ADAM33 (Human) ELISA Kit | E5102-100

Description: ADAM33 is a member of the ADAM (a disintegrin and metalloproteinase) gene family and have been implicated in a variety of biological processes involving cell–cell and cell–matrix interactions, including fertilization, muscle development, and neurogenesis. This protein is a type I transmembrane protein implicated in asthma and bronchial hyperresponsiveness. Alternative splicing of this gene results in two transcript variants encoding different isoforms. The preferential expression of ADAM33 in mesenchymal cells and its multiple molecular actions provide ample opportunity for incriminating this molecule in chronic asthma. The identification of ADAM33 as a major risk factor involved in the pathogenesis of bronchial hyperresponsiveness and airway wall remodeling provides insight into the pathogenesis of asthma and represents a novel therapeutic target. ADAM33 also acts as a novel molecular biomarker of Triple-negative breast cancer BLBC that might be useful as a prognostic factor. ’s ADAM33 (Human) ELISA Kit is used for the quantitative detection of ADAM33 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of ADAM33 in the sample.

Alternate Name: ADAM33 ELISA Kit; Disintegrin and metalloproteinase domain-containing protein 33 ELISA Kit; ADAM 33 ELISA Kit; ADAM metallopeptidase domain 33 ELISA Kit; C20orf153 ELISA Kit; chromosome 20 open reading frame 153 ELISA Kit; disintegrin and metalloproteinase dain-containing protein 33 ELISA Kit; Disintegrin and reprolysin metalloproteinase family protein ELISA Kit

Tag Line: A highly sensitive sandwich ELISA kit for the quantitative measurement of ADAM33

Summary: N/A

Detection Method: Absorbance is measured at 450 nm

Sample Type: Human Serum, Plasma, Tissue lysates and other biological fluids

Species Reactivity: Human

Applications: The ELISA kit is used for the quantitative detection of ADAM33 in serum, plasma, tissue homogenates and other biological fluids

Features & Benefits: Sensitivity: 46.875 pg/ml Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10% Highly sensitive and specific Recovery Rate: 87-102% for serum, 89-102% for EDTA plasma and 90-105% for heparin plasma Detection range: 78.125-5000 pg/ml