Adenosine kinase, Human Recombinant | P1234

Adenosine kinase, also known as ADK is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Recombinant human Adenosine kinase, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

P1234 | Adenosine kinase Human Recombinant DataSheet

Biomolecule/Target: ADK

Synonyms: ADK, AK

Alternates names: ADK, AK

Taglines: Potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides

NCBI Gene ID #: 20315

NCBI Gene Symbol: SDF1A

Gene Source: Human

Accession #: P40224

Recombinant: Yes

Source: E.coli

Purity by SDS-PAGEs: 99%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to convert 1.0 pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.

Biological activity: Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 50-100 ng/ml.

Results: > 30 pmol/min/ug

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 7.9 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 20% glycerol, 1 mM EDTA, 50 mM NaCl

Reconstitution Instructions: We recommend a quick spin followed by reconstitution in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK